Wepredic
Scientific Resources HepaSH™
Application of HepaSH cells for predicting cholestatic drug-induced liver injury via bile acid-dependent toxicity assay
Takemura A, Naito S, Higuchi Y, Uehara S, Yoneda N, Suemizu H, Ito K; 2026
Lentiviral-mediated expression of cytochrome P450 2D6 in HepaRG cells: new means for in vitro xenobiotic biotransformation and cellular response to chimeric viral mRNA.
Coppens-Exandier H, Mackanga F, Darde T, Bonhomme C, Angenard G, Leroyer P, Ribault C, Gicquel T, Pelletier R, Roshchina F, Schaefer E, Chesné C, Jamin A, Corlu A, Loyer P; 2026
Regulatory impact of G-quadruplexes in the human carboxylesterase 2 gene on drug and lipid metabolism
Kuroda S, Nakashima S, Takemoto S, Nakano M, Higuchi Y, Uehara S, Yoneda N, Suemizu H, Nakajima M, Fukami T; 2026
Development of a DNA damage assay system using stable human hepatocytes
Mishima M, Izawa K, Tsuda M, Higuchi Y, Uehara S, Suemizu H, Sugiyama KI; 2026
Auranofin, identified by FDA-approved drug library screening, inhibits HBs antigen secretion via lysosomal damage
Shimoda A, Murai K, Hikita H, Minami S, Miyake T, Kuriki S, Sometani E, Sung J, Shigeno S, Higuchi Y, Maesaka K, Shirai K, Tahata Y, Saito Y, Kodama T, Takahashi T, Suemizu H, Takehara T; 2026
Prediction of human hepatic clearance with HepaSH monolayers for low-turnover compounds
Shotaro Uehara, Yuichiro Higuchi, Nao Yoneda, Hiroshi Yamazaki, Hiroshi Suemizu; 2025
3D hepatic spheroids and liver‐organ on chip models displayed maintenance of hepatic functions and maturation profile in a long‐term culture of the humanized HepaSH cells
Hanyuan Wang, Carla Meschini, Stéphane Poulain, Soo Hyeon Kim, Hiroshi Arakawa, Yukio Kato, Cecile Legallais, Ulysse Pereira, Yasuyuki Sakai, Eric Leclerc; 2025
Palmitic acid induced the onset of lipotoxicity in a HepaSH-on-chip model with raised of H2O2 and IL-6, and altered p38/MAPK & JAK/ STAT pathways
Hanyuan Wang , Stéphane Poulain , Wenxin Cao, Hiroshi Arakawa , Soo Hyeon Kim , Yukio Kato , Masaki Nishikawa, Yasuyuki Sakai , Eric Leclerc; 2025
In Vitro Assessment of Drug-Induced Liver Injury Using Three-Dimensional Cultured HepaSH Cells Derived From Chimeric Mouse Model With Humanized Liver
Liu X, Higuchi Y, Sakamoto Y, Yanagida S, Uehara S, Suemizu H, Kanda Y, Kawagishi H; 2025
Human aldo-keto reductase (AKR) 1C1 and 1C2 act as coactivators of pregnane X receptor, a master regulator of drug-metabolizing and gluconeogenesis enzymes
Mitamura R, Takemoto S, Aoyama Y, Morita K, Higuchi Y, Uehara S, Yoneda N, Suemizu H, Fukami T, Nakano M, Nakajima M; 2025
Exploiting metabolic vulnerability in glioblastoma using a brain-penetrant drug with a safe profile
Burban A, Tessier C, Larroquette M, Guyon J, Lubiato C, Pinglaut M, Toujas M, Galvis J, Dartigues B, Georget E, Luchman HA, Weiss S, Cappellen D, Nicot N, Klink B, Nikolski M, Brisson L, Mathivet T, Bikfalvi A, Daubon T, Sharanek A; 2025
Induction of drug metabolizing enzyme and drug transporter expression by antifungal triazole pesticides in human HepaSH hepatocytes
Zerdoug A, Le Vée M, Le Mentec H, Carteret J, Jouan E, Jamin A, Lopez B, Uehara S, Higuchi Y, Yoneda N, Chesné C, Suemizu H, Fardel O; 2025
Impact of miR-222-3p-mediated downregulation of arylacetamide deacetylase on drug hydrolysis and lipid accumulation
Sakai Y, Fukami T, Tokumitsu S, Nakano M, Nakashima S, Higuchi Y, Uehara S, Yoneda N, Suemizu H, Nakajima M; 2024
In Vivo and In Vitro Induction of Cytochrome P450 3A4 by Thalidomide in Humanized-Liver Mice and Experimental Human Hepatocyte HepaSH cells
Uehara S, Murayama N, Higuchi Y, Shimizu M, Suemizu H, Guengerich FP, Yamazaki H; 2024
Cytochrome P450 1A2 and 2C enzymes autoinduced by omeprazole in dog hepatocytes and human HepaRG and HepaSH cells are involved in omeprazole 5-hydroxylation and sulfoxidation
Yasuhiro Uno, Shotaro Uehara, Genki Ushirozako, Norie Murayama, Hiroshi Suemizu, Hiroshi Yamazaki; 2023
Drug transporter expression and activity in cryopreserved human hepatocytes isolated from chimeric TK-NOG mice with humanized livers.
Zerdoug A, Le Vée M, Uehara S, Jamin A, Higuchi Y, Yoneda N, Lopez B, Chesné C, Suemizu H, Fardel O; 2023
HepaSH cells: Experimental human hepatocytes with lesser inter-individual variation and more sustainable availability than primary human hepatocytes
Uehara S, Higuchi Y, Yoneda N, Ito R, Takahashi T, Murayama N, Yamazaki H, Murai K, Hikita H, Takehara T, Suemizu H; 2023